Manual Solid–Phase Peptide Synthesis of Metallocene–Peptide Bioconjugates

Abstract

We describe a simple procedure for the manual solid-phase synthesis of peptides. No expensive or specialized equipment is needed. Moreover, a variety of markers can be attached to the N-terminus. We describe two organometallic markers and a fluroescent dye, in addition to an unlabeled derivative that serves as a comparison. The experiment described herein was successfully carried out by fourth-year undergraduate students of molecular biotechnology. By carrying out this experiment, the students gained first-hand experience for one of the most important modern synthesis techniques.