Manual polybrene test and micro-column gel test in clinical blood transfusion: application research

Abstract

Objective To investigate the application of manual polybrene test (MPT) and micro-column gel test (MGT) in clinical blood cross-matching and irregular antibody screening of blood samples. Methods A total of 1 386 cases of transfusion patients in Shandong Provincial Traffic Hospital from August 2012 to August 2014 were selected as research objects. MPT and MGT were adopted respectively to perform irregular antibody screening. Panel cells were adopted to perform antibody identification in the positive results. The blood cross-matching test time, results and false positive reasons of MPT and MGT, and irregular antibody screening results were observed. The blood cross-matching test time, positive rates of the primary side and the secondary side, total positive rate, false positive rate and irregular antibody screening sensitivity of MPT and MGT were statistically compared. This study was agreed by the Human Trials Committee of Shandong Provincial Traffic Hospital, and signed the informed consents with all the patients. Results ①The blood cross-matching time of MPT was (12.1±2.6) min, it was obviously shorter than that of MGT which was (33.7±3.1) min, and the difference was statistically significant (t=198.83, P 0.05). The secondary side positive rate of MPT was 0.14% (2/1 386), which was significantly lower than that of MGT (0.65%, 9/1 386), and the difference was statistically significant (χ2=4.47, P=0.034). ③The irregular antibody screening sensitivity of MPT and MGT was 78.95% (15/19) and 100.00% (19/19), respectively, and there was significant difference between them (χ2=4.47, P=0.034). Conclusions The screening sensitivity of MPT in detection of irregular antibodies is higher than that of MPT, but it need more time than that of MPT. So as to clinical blood transfusion compatibility test, the combination of these two methods can be used to reduce cross matching time, and improve clinical transfusion safety at the same time. Key words: Blood grouping and crossmatching; Manual polybrene test; Micro-column gel test; Antibodies, irregular