Manual microcolumn chromatography for sample cleanup before mass spectrometry.

Abstract

BioTechniquesVol. 22, No. 2 BenchmarksOpen AccessManual Microcolumn Chromatography for Sample Cleanup Before Mass SpectrometryPaul L. Courchesne & Scott D. PattersonPaul L. CourchesneAmgen, Thousand Oaks, CA, USASearch for more papers by this author & Scott D. Patterson*Address correspondence to Scott D. Patterson, Amgen, Amgen Center, Mail Stop 14-2-E, 1840 DeHavilland Drive, Thousand Oaks, CA 91320-1789, USA. Internet: E-mail Address: spatters@amgen.comAmgen, Thousand Oaks, CA, USASearch for more papers by this authorPublished Online:7 Aug 2018https://doi.org/10.2144/97222bm10AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByHigh-Throughput Glycomic Methods7 July 2022 | Chemical Reviews, Vol. 19Negative detection of biomolecules separated in polyacrylamide electrophoresis gels9 November 2006 | Nature Protocols, Vol. 1, No. 3Suppression of α-Cyano-4-hydroxycinnamic Acid Matrix Clusters and Reduction of Chemical Noise in MALDI-TOF Mass Spectrometry13 April 2004 | Analytical Chemistry, Vol. 76, No. 10Direct Atmospheric Pressure Coupling of Polyacrylamide Gel Electrophoresis to Mass Spectrometry for Rapid Protein Sequence Analysis29 July 2003 | Journal of Proteome Research, Vol. 2, No. 6Stop and Go Extraction Tips for Matrix-Assisted Laser Desorption/Ionization, Nanoelectrospray, and LC/MS Sample Pretreatment in Proteomics24 December 2002 | Analytical Chemistry, Vol. 75, No. 3Chapter 29 Sample cleanup and concentration techniques for capillary electrophoresisA simplified device for protein identification by microcapillary gradient liquid chromatography-tandem mass spectrometryElectrophoresis, Vol. 21, No. 9Better approaches to finding the needle in a haystack: Optimizing proteome analysis through automationElectrophoresis, Vol. 21, No. 6Protein identification methods in proteomicsElectrophoresis, Vol. 21, No. 6Protein Micro-Characterization by Mass Spectrometry: Sample Handling and Data FlowTechniques for sample preparation including methods for concentrating peptide samplesIdentification of Proteins by Mass SpectrometryExamination of micro-tip reversed-phase liquid chromatographic extraction of peptide pools for mass spectrometric analysisJournal of Chromatography A, Vol. 826, No. 2Introduction Vol. 22, No. 2 Follow us on social media for the latest updates Metrics Downloaded 78 times History Published online 7 August 2018 Published in print February 1997 Information© 2018 Author(s)PDF download