Abstract
This article demonstrates that mannotriose effectively induces the differentiation of mesenchymal stem cells into neuron-like cells in vitro. Rat-derived mesenchymal stem cells were investigated on their potential to differentiate into neuron-like cells induced by mannotriose purified from Radix Rehmanniae Preparata in vitro. The percentage of the neuron-specific enolase positive cells and the Nissl positive cells after mannotriose treatment was increased. The mRNA levels of neurofilament medium and neuron-specific enolase were upregulated in the mannotriose group compared to the control. These findings demonstrate that mannotriose purified from Radix Rehmanniae Preparata can effectively induce differentiation of rat-derived mesenchymal stem cells into neuron-like cells.
Highlights
Bone mesenchymal stem cell (BMSC) is a bone marrowderived multipotent stem cell, which can differentiate into several somatic cells under certain circumstances, such as osteoblasts, chondrocytes, adipocytes
The induction of Mesenchymal stem cells (MSC) to differentiate into neurons for cell therapy provides a new possibility for the treatment of neurological disorders and have been proposed as an alternative therapy to be applied to several pathologies of the nervous system [6,7,8,9,10]
We found that most neuron-like cells treated with mannotriose (0.1 g/L) for 48 h were positive for Nissl staining (Fig. 5)
Summary
Bone mesenchymal stem cell (BMSC) is a bone marrowderived multipotent stem cell, which can differentiate into several somatic cells under certain circumstances, such as osteoblasts, chondrocytes, adipocytes. Mesenchymal stem cells (MSC) could differentiate into neuron-like cells and glialike cells [1,2,3,4,5]. They have abundant sources and could be isolated with high yield, which added to their ability to differentiate into non-mesodermal cell types, including neuronal lineage in vivo and in vitro. Radix Rehmanniae Preparata-containing serum can induce BMSCs into neural cell differentiation [11]. Whether mannotriose is toxic for the quality control of Radix Rehmanniae Preparata needs further study. Mannotriose is natural, and Radix Rehmanniae Preparata could effectively treat neurologic diseases [14, 15]. It is imperative to find safer alternatives [16] validated
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