Mannose-Induced Modulations in Antioxidants, Protease Activity, Lipid Peroxidation, and Total Phenolics in Etiolated Wheat Leaves

Abstract

Modulation of different antioxidants, total phenolics, lipid peroxidation, and protease activity as a result of mannose treatment (1%) was studied in leaves of etiolated wheat seedlings. Changes in these biochemicals were monitored up to 96 h after treatment at 24-h intervals. Mannose treatment induced a significant increase in protease activity throughout the scanning period, coupled with a gradual decrease in leaf protein content. Membrane lipid peroxidation (MDA content) was higher at 24 and 72 h after treatment. MDA content remained higher for a longer period due to mannose treatment. During the initial 24 h of mannose treatment, only catalase and total phenolic contents were increased. Catalase activity was down regulated with increasing duration of treatment. On the other hand, peroxidase (POD, APX) activities were initially unaffected but increased with increasing treatment duration. The decreased level of lipid peroxidation at 96 h may be due to detoxification of H2O2 by peroxidases. Superoxide dismutase activity was not affected by mannose treatment. In conclusion, evidence is provided that mannose can modulate the expression of the enzymatic antioxidant defense system in wheat leaves.