Abstract
To determine the effect of sperm manipulation before intracytoplasmic sperm injection (ICSI) on fertilization rates. Three methods of sperm manipulation before direct ICSI were compared in two sibling oocyte trials. In vitro fertilization unit within a teaching hospital. Patients undergoing infertility treatment using ICSI. Oocytes were inseminated by ICSI. Fertilization rate. In the first trial, a standard manipulation technique gave a 67% fertilization rate compared with 64% with a minimal manipulation technique. In the second trial the standard technique gave a significantly greater fertilization rate compared with no prior manipulation (67% versus 45%). Manipulation of sperm before direct ICSI is not mandatory for fertilization but significantly improves the fertilization rate.
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