Abstract
The addition of 25-azacycloartanol to the medium of suspension cultures of bramble cells resulted, after 6 weeks of growth, in a large decrease in the percentage of C 10 side-chain sterols, sitosterol and isofucosterol (83 % of the total in the control, 9 % in the treated cells), and in a spectacular increase in the percentage of C 8 side-chain sterols, cycloartenol, desmosterol and cholesterol (less than 1 % in the control, 53 % in the treated cells). In addition the relative percentage of C 9 side-chain sterols, mainly 24-methylene cholesterol increased significantly (from 16 to 37 %). A secondary effect of 25-azacycloartanol consisted in an increase of the percentage of Δ 24 sterols and in a decrease of the percentage of sterols with a saturated side chain. These results are in agreement with an inhibition by 25-azacycloartanol of the C-24 and C-28 methyltransferases and of the Δ 24 reductase.
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