Abstract
Publisher Summary This chapter examines the manipulation and analysis of polyketide (PKS) synthases. PKS span a large range of medicinally important com-pounds, such as antibiotics, anticancer agents, immunosuppressants, and cholesterol-lowering agents. PKS are produced in nature by micro-organisms and may have suboptimal properties for their appropriate usage. The characterization and engineering of type I PKS had, for the most part, been performed in natural producing organisms. Both starter unit and extender unit specificities have been modified in PKS producing actinomycetes using homologous recombination. In addition to propionate, the modified PKS inserted a large assortment of Ī± -branched starter units into 6- deoxyerythronolide B synthase. Accessory proteins were able to transform the unnatural aglycons into the corresponding erythromycin A analogs. The PKS clusters involved in the biosynthesis of actinorhodin and undecylprodigiosin pigment were deleted from the chromosome using homologous recombinations. The resulting strain is effectively void of major PKS production. It is found that CH999 has been instrumental in the elucidation of individual protein functions in the type II PKS systems.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
