Abstract
Low-power laser irradiation of red light has been recognized as a promising tool across a vast variety of biomedical applications. However, deep understanding of the molecular mechanisms behind laser-induced cellular effects remains a significant challenge. Here, we investigated mechanisms involved in the death process in human hepatic cell line Huh7 at a laser irradiation. We decoupled distinct cell death pathways targeted by laser irradiations of different powers. Our data demonstrate that high dose laser irradiation exhibited the highest levels of total reactive oxygen species production, leading to cyclophilin D-related necrosis via the mitochondrial permeability transition. On the contrary, low dose laser irradiation resulted in the nuclear accumulation of superoxide and apoptosis execution. Our findings offer a novel insight into laser-induced cellular responses, and reveal distinct cell death pathways triggered by laser irradiation. The observed link between mitochondria depolarization and triggering ROS could be a fundamental phenomenon in laser-induced cellular responses.
Highlights
Light plays a crucial role in important biological processes directly related to a human health such as: vision, vitamin-D metabolism, circadian rhythm and psychosocial state
Burst of research in this direction resulted in therapeutic applications of low power red and near-infrared light to promote wound healing, hair growth, tissue regeneration or reduce pain and inflammation termed as Low Level Light/Laser Therapy (LLLT) [3, 6, 11,12,13]
Power levels ranged from 50 to 200 mW are used in LLLT and from 6 to 15 W are applied at high power NIR phototherapy [41]
Summary
Light plays a crucial role in important biological processes directly related to a human health such as: vision, vitamin-D metabolism, circadian rhythm and psychosocial state. LLLT became widely used to treat a variety of ailments, it remains controversial [14,15,16] These inconsistencies in the literature are not surprising. Despite that the biological effects of low power red and near-infrared (NIR) radiation have been studied for decades, its underlying biochemical mechanisms remain poorly understood. Effects of low power red and near-infrared light on biological responses and safety have not been investigated thoroughly. Only the knowledge of the spatiotemporal mechanisms of the laser-induced effects will enable the deliberate exploitation of cellular signaling processes. Such knowledge is crucial for the development of well-controlled laser applications to achieve a significant therapeutic benefit
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
