Manipulating osa-MIR156f Expression by D18 Promoter to Regulate Plant Architecture and Yield Traits both in Seasonal and Ratooning Rice

Qing Liu,Langtao Xiao,Bin Hong,Keqin Peng,Yi Su,Yunhua Zhu,Ruozhong Wang,Mahmoud Gaballah

doi:10.1186/s12575-019-0110-4

Qing Liu, Langtao Xiao + Show 6 more

Open Access

https://doi.org/10.1186/s12575-019-0110-4

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Abstract

BackgroundRice (Oryza sativa L.) feeds more than half of the world’s population. Ratooning rice is an economical alternative to the second seasonal rice, thus increasing the yield of ratooning rice is highly important.ResultsHere we report an applicable transgenic line constructed through the manipulation of osa-MIR156f expression in rice shoot using the OsGA3ox2 (D18) promoter. In seasonal rice, the D18–11 transgenic line showed moderate height and more effective tillers with normal panicle. In ratooning rice, axillary buds outgrew from the basal node of the D18–11 transgenic line before the harvest of seasonal rice. More effective tillers produced by the outgrowth of axillary buds contributed to the plant architecture improvement and yield increase. Additionally, it was found that osa-miR156f down-regulated the expression of tillering regulators, such as TEOSINTE BRANCHED1 (TB1) and LAX PANICLE 1 (LAX1). The expression of DWARF10, DWARF27 and DWARF53, three genes being involved in the biosynthesis and signaling of strigolactone (SL), decreased in the stem of the D18–11 transgenic line.ConclusionOur results indicated that the manipulation of osa-MIR156f expression may have application significance in rice genetic breeding. This study developed a novel strategy to regulate plant architecture and grain yield potential both in the seasonal and ratooning rice.

Highlights

Rice (Oryza sativa L.) feeds more than half of the world’s population
Osa-MIR156f was expressed in the rice stem under the control of the promoter of a GA biosynthesis gene, GA3ox2 (D18) [46, 47]
We have identified that osa-MIR156f plays important roles in rice tillering and plant architecture [38]

Summary

Introduction

Rice (Oryza sativa L.) feeds more than half of the world’s population. Rice (Oryza sativa L.) is the staple food of more than half of the world’s population which is expected to reach 9 billion by the year of 2050. Increasing crop yield is one of the most important goals in modern agriculture. The ideal plant architecture for rice, i.e. the phenotype of moderate height, enough effective tillers, large panicle and robust stems/roots, is crucial for high yield [3, 4]. Liu et al Biological Procedures Online (2019) 21:21 genes being related to gibberellin (GA) biosynthesis/signaltransduction, jointly regulate plant height [17]. The successful identification of these genes involved in rice plant architecture regulation greatly promoted the elucidation of the underlying molecular mechanisms

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