Mango agro-industrial wastes for lipase production from Yarrowia lipolytica and the potential of the fermented solid as a biocatalyst

Abstract

Mango seed and peel, wastes from the industrial processing of the fruit, were evaluated for lipase production by Yarrowia lipolytica. Submerged fermentation of the components of mango wastes (peel, tegument and kernel) were performed with the components separately and their combinations without any supplementation. The tegument was the only part that showed promising results for lipase production and, therefore several nitrogen sources were used, separately, as supplementation. Yeast extract was the best nitrogen source for lipase production with mango tegument in submerged fermentation, achieving around 3500U/L of extracellular lipase. Optimum conditions for lipase production were pH 5.0; 187rpm of agitation speed; temperature of 27.9°C and inoculum concentration of 0.96g/L. Lipase activity of the solid residue obtained after fermentation carried out in the best experimental conditions was 68.03U/(g of residue) evidencing that the lipase produced during fermentation adsorbed in the fermented solid, producing a new biocatalyst.