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Abstract
The study evaluates the protective effect of mangiferin on osteosarcoma cell proliferation and metastasis. Saos-2 and U2OS cells were treated with mangiferin (25, 50, 75 and 100 µM) for 72 h. Mangiferin reduced the cell viability, invasion, and cell adhesion and migration rate. Matrix metalloproteinases-2/9 (MMP-2/9) mRNA expression was reduced significantly, while the levels of tissue inhibitors of metalloproteinases-1/2 (TIMP-1/2) were elevated in Saos-2 and U2OS cells. Mangiferin treatment significantly reduced parathyroid hormone receptor 1 (PTHR1) mRNA and protein expression by more than 0.5-fold in both osteosarcoma cells. In addition, the immunofluorescent analysis also showed decreased PTHR1 expression following treatment with mangiferin. In summary, we have demonstrated that treatment with mangiferin reduces cell viability, proliferation, invasion, adhesion and migration, and induces apoptosis of osteosarcoma cells. Therefore, treatment with mangiferin can be effective agent in inhibiting growth and inducing apoptosis in osteosarcoma cells. Our experimental results provide evidence for the therapeutic effect of mangiferin in osteosarcoma cells.
Highlights
Osteosarcoma is severe malignant bone tumor (Li et al 2018), and teenagers and adults are affected mostly by osteosarcoma (Luetke et al 2014)
Effect of mangiferin on osteosarcoma cell viability We investigated the impact of various concentrations of mangiferin on the invasion and proliferation of Saos-2 and U2OS cells following 72 h of treatment
Mangiferin decreased the proliferative potential of U2OS cells to 10.8%, 30.8%, Effect of mangiferin on osteosarcoma cell detachment and cell adhesion Mangiferin increased the percent of detached Saos-2 cells to 9.1%, 26.2%, 37.4% and 46.2% at 25, 50, 75 and 100 μM, respectively (Fig. 4a, c), whereas mangiferin increased U2OS cell detachment to 13%, 25%, 33% and 48% at 25, 50, 75 and 100 μM, respectively (Fig. 4b, c)
Summary
Osteosarcoma is severe malignant bone tumor (Li et al 2018), and teenagers and adults are affected mostly by osteosarcoma (Luetke et al 2014). Ottaviani and Jaffe (2009) have reported the increased mortality rate in childhood due to malignant bone and joint tumor. Chemotherapy and surgical resection have improved the survival rate of osteosarcoma up to 70%. Osteosarcoma recurrence and metastasis leads to an increased mortality rate Dar et al (2005) have reported the several pharmacological effects of mangiferin such as antioxidant, anticancer, antiaging, antiviral, hepatoprotective, analgesic and immunomodulatory potential. The study analyzed the ability of mangiferin suppresses human metastatic osteosarcoma cell growth by down-regulating the expression of MMP2/9 and PTHR1
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