MANGIFERIN PROTECTS RENAL IMPAIRMENT AGAINST BENZO(A)PYRENE INDUCED TOXICITY BY REGULATING MITOCHONDRIAL AND DNA INTEGRITY

Abstract

Contaminated food and water ingestion and inhalation of polycyclic aromatic hydrocarbon are the main sources of human exposure to benzo(a)pyrene leading to cause renal injury and provoking a nephropathic response. Mangiferin is a highly potent antioxidant present in mango and known for several medicinal properties. The present study was undertaken to evaluate the renoprotective effect of mangiferin against benzo(a)pyrene induced toxicity. Benzo(a)pyrene was administered at a dose of 120.0 mg/kg once alone or in combination with mangiferin at 10.0 and 20.0 mg/kg for seven days in Swiss albino mice. Exposure to benzo(a)pyrene decreased the activities of glutathione peroxidase and increased glutathione-S-transferase level in the kidneys of mice. Moreover, DNA alkaline unwinding assay exhibit a significant decrease in F-value for the toxic control group in comparison to the normal mice, which is a marker for alteration in DNA integrity. Benzo(a)pyrene treatment also revealed an increased number of micronuclei in polychromatic erythrocytes in comparison with the control group indicating chromosomal damage in erythrocytes of bone marrow. Mangiferin pretreatment significantly improved the renal mitochondrial antioxidant status and restored the renal DNA integrity, thus demonstrating the protective effect in benzo(a)pyrene-treated mice. Dietary inclusion of mangiferin could exert protective effects against renal toxicity resulting from benzo(a)pyrene exposure.Keywords: Benzo(a)pyrene, GPx, GST, Mangiferin, Micronucleus Â