Abstract
To investigate the anti-proliferative mechanism of mangiferin in a human nasopharyngeal carcinoma cell line, CNE2 cells were incubated with different concentrations of mangiferin (12.5, 25, 50, 100, 150 and 200 μM) or with PBS as a control for 72 hours. Analyses were made of the cell cycle and apoptosis with measurement of mRNA and protein levels of two apoptosis-related genes, Bcl-2 and Bax. Flow cytometry assays showed mangiferin could inhibit CNE2 cell proliferation via G2/M arrest and induction of early apoptosis. Real time PCR and Western blotting showed the mRNA and protein level of Bcl-2 to be down-regulated, while those of Bax were up-regulated, when CNE2 cells were treated with mangiferin. This investigation indicated anti-proliferation effects of mangiferin through induction of cell apoptosis regulated by Bcl-2 and Bax expression.
Highlights
Mangiferin, a C-glucosyl xanthone (1, 3, 6, 7 -tetrahydroxyxanthone -C2 -β -D -glucoside), is a natural flavonoid compound widely distributed in a variety of plant families, such as Mangifera indica, Mangifera odorata, Gentiana lutea, Pyrrosia gralla and Rhizoma anemarrhenae
Cell apoptosis induced by mangiferin Cell apoptosis and cell cycle phases was analysed by flow cytometry using annexin V-PE/Propidium iodide (PI) staining
We found that mangiferin could induce CNE2 cells apoptosis in a dose
Summary
Mangiferin, a C-glucosyl xanthone (1, 3, 6, 7 -tetrahydroxyxanthone -C2 -β -D -glucoside), is a natural flavonoid compound widely distributed in a variety of plant families, such as Mangifera indica, Mangifera odorata, Gentiana lutea, Pyrrosia gralla and Rhizoma anemarrhenae. The easiest source of mangiferin is mango plant (Mangifera indica), especially the leaves. The mango tree is indigenous to the Indian. It was introduced into China in the Tang Dynasty, has been cultivated in South China for over 1000 years (Wang et al, 1997). The chemistry of this nature compound, mangiferin, has been relatively well understood. Previous studies demonstrated that it exhibit numerous biological activities, including anti-inflammatory, anti-proliferative, antioxidative, anti-diabetic and neuroprotective effects (Ichiki et al, 1998; Yoshimi et al, 2001; Muruganandan et al, 2002)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
