Manganese removal and product characteristics of a marine manganese-oxidizing bacterium Bacillus sp. FF-1.

Abstract

Biogenic manganese oxides (BioMnOx) have been found all over the world, and most of them were formed by Mn(II)-oxidizing bacteria (MnOB). In this study, a MnOB designated as FF-1 was isolated from marine surface sediments in the Bohai Sea, China. This strain was identified as Bacillus sp. and can tolerate more than 5% salinity. It can grow in the presence of 0-7mM Mn(II) and pH range from 5.0 to 7.0. When the initial Mn(II) was 5mM, the percentage of Mn(II) oxidation reached the highest value of 16% after 10days of incubation. The initial pH (5.0 to 7.0) affected the percentage of Mn(II) oxidation, but the ability of the strain FF-1 to self-regulate pH resulted in the final pH being almost 7.6. The removal of Mn(II) by the strain FF-1 involves extracellular and intracellular adsorption as well as Mn(II) oxidation. Intracellular Mn adsorption contributed a small part to the total Mn removal, and extracellular adsorption was dominant in the initial stage of Mn removal. The solid products after Mn removal were a mixture of MnOx and MnCO3. The layered MnOx formed in the extracellular space could be easily collected and used for adsorption and oxidation of pollutants.