Manganese content in some organs and tissues of Landrace pigs

Abstract

In the article, the authors presented the results of assessing the level of manganese in the skeletal muscles, liver, kidneys, and bristles of Landrace pigs. The studies were performed on clinically healthy six-monthold animals from a large pig farm in the Altai Territory. The conditions of keeping the animals corresponded to the standard ones, the feeding was typical for meat fattening. Elemental analysis of samples of parenchymal organs and muscle tissue was performed using atomic absorption spectrometry with flame and electrothermal atomization, bristles - by atomic emission spectrometry with inductively coupled plasma. Data processing was performed using Microsoft Office Excel and the R programming language in the data analysis environment RStudio version 1.3.1093 (2009–2020 RStudio, PBC). The authors found that the distribution in all cases was normal, and the dispersions are not homogeneous. Based on the average value and median, an increasing ranked series of manganese content in organs and tissues were established: skeletal muscles < kidneys < liver < bristles, in numerical terms: 1: 12.5: 15.7: 207. Manganese levels in skeletal muscles, kidneys, liver, and bristles were 0.13; 1.62; 2.04, and 26.91 mg/kg, respectively. Greater homogeneity is characteristic of the accumulation of microelements in the muscles and kidneys. Based on the Kruskal-Wallis test, it was established that the accumulation of manganese differs in the examined organs and tissues of pigs (H = 88.4, df = 3, p < 0.0001). Pairwise comparison showed significant differences for all pairs, except for the liver-kidney pair. Based on the cluster analysis, the closest results are typical for the liver and kidneys; the bristle is a separate opposite cluster, characterized by a significant level of manganese accumulation. The results obtained can serve as a preliminary corresponding physiological norm for the concentration of manganese in individual organs, tissues, and skin derivatives of Landrace pigs under the conditions of Western Siberia.