MAMP-triggered resistance induced by elicitor protein PeBA1 derived from Bacillus amyloliquefaciens NC6 in common bean (Phaseolus vulgaris L.) against green peach aphid (Myzus persicae Sulzer)

Abdul Basit,Yong Wang,Muhammad Farhan,Abdul Hanan,Muhammad Z Majeed,Tala Nazir,Dewen Qiu

doi:10.15835/nbha48211901

Abstract

Bacterial microbe-associated molecular patterns (MAMPs) play an important role in innate plant immunity. This in vitro study evaluated the putative role of protein elicitor PeBA1 derived from Bacillus amyloliquefaciens NC6 strain in eliciting induced resistance type responses in common bean (Phaseolus vulgaris) plants against green peach aphid Myzus persicae. Nymphal developmental time of aphids was significantly prolonged and the fecundity was significantly reduced by different concentrations of PeBA1 elicitor (i.e. 40.51, 24.91 and 16.38 µg mL-1) applied at three different temperature regimes (i.e. 21, 27 and 30 °C). Moreover, foliar application of PeBA1 elicitor protein strongly up-regulated the expression levels of salicylic acid (SA) pathway-associated genes, while the expression levels of jasmonic acid (JA) pathway-associated genes exhibited a moderate induction. Quantification by LC/MSMS revealed a linear increase of both SA and JA plant defense hormones along with the time of exposure. Our findings suggest that the bacterial elicitor protein PeBA1 could be used as an effective biological pest management tool against phloem-feeding insect pests such as green peach aphids M. persicae.

Highlights

In nature, plants are confronted by a wide range of pathogens and herbivore pests
Influence of PeBA1 elicitor on nymphal development time of aphids Analysis of variance showed a significant impact of different concentrations of PeBA1 elicitor protein (F3, 468 = 12.71; p < 0.001), temperature regimes (F2, 468 = 274.27; p < 0.001) and of their interaction (F6, 468 = 5.61; p < 0.001) on the overall developmental time of M. persicae (Table S2)
We postulated that the application of PeBA1 elicitor would prolong the nymphal development time and reduce the fecundity of M. persicae on common bean plants

Summary

Introduction

Plants are confronted by a wide range of pathogens and herbivore pests In response to these biotic stresses, they have evolved different physiological and molecular defense mechanisms including hypersensitive response (HR), defense signaling pathways, reactive oxygen species (ROS) and reactive nitrogen. Received in revised form: 08 May 2020. Not Bot Horti Agrobo 48(2):705715 species (RNS) synthesis (Delledonne et al, 2001; Garcia-Brugger et al, 2006) These plant defense pathways are regulated by transcriptional and metabolic changes through physiological reactions, and are often directly or indirectly activated by different signaling molecules such as nitric oxide (NO) and ROSs produced by the activation of plasma membrane proteins or protein phosphorylation (Garcia-Brugger et al, 2006)

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Conclusion