Abstract
Genetic Engineering & Biotechnology NewsVol. 32, No. 17 TutorialMammalian Transient Transfection SystemNovel Approach Produces Gram-Per-Liter Expression Levels of Biologically Active ProteinsMeredith B. Jones, Chao Yan Liu, Sanjay Vasu, Isabel Cisneros, Henry Chiou, and Jonathan F. ZmudaMeredith B. Jones*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this author, Chao Yan Liu*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this author, Sanjay Vasu*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this author, Isabel Cisneros*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this author, Henry Chiou*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this author, and Jonathan F. Zmuda*Meredith B. Jones, Ph.D., *Chao Yan Liu, *Sanjay Vasu, Ph.D., and *Isabel Cisneros are scientists, Henry Chiou, Ph.D. (E-mail Address: Henry.Chiou@lifetech.com), is a senior product manager, and Jonathan F. Zmuda, Ph.D., is an associate director, R&D at Life Technologies. The authors would like to thank Yolanda Tennico for her expert assistance with glycosylation profiling. The Expi293 Expression System and FreeStyle 293 Expression System are for Research Use Only. Not for use in diagnostic procedures. *These authors contributed equally to this work.Search for more papers by this authorPublished Online:28 Sep 2012https://doi.org/10.1089/gen.32.17.21AboutSectionsView articleView Full TextPDF/EPUB ToolsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail View articleFiguresReferencesRelatedDetailsCited ByEpitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit CitrullinationJournal of Immunology Research, Vol. 2021Transient expression of human antibodies in mammalian cells14 December 2017 | Nature Protocols, Vol. 13, No. 1Efficient and inexpensive transient expression of multispecific multivalent antibodies in Expi293 cells15 September 2017 | Biological Procedures Online, Vol. 19, No. 1 Volume 32Issue 17Oct 2012 Information© 2012 by GEN PublishingTo cite this article:Meredith B. Jones, Chao Yan Liu, Sanjay Vasu, Isabel Cisneros, Henry Chiou, and Jonathan F. Zmuda.Mammalian Transient Transfection System.Genetic Engineering & Biotechnology News.Oct 2012.50-51.http://doi.org/10.1089/gen.32.17.21Published in Volume: 32 Issue 17: September 28, 2012PDF download
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