Mammalian model based histological comparison of fresh and expired mecobalamin intake on liver and kidney

Abstract

This study was designed to estimate the possible histological effects of fresh and expired mecobalamin regular exposure to hepatic and renal tissues after 4 weeks. For this purpose, thirty male albino mice of 8 weeks age were kept in animal house for 10 days habituation and then they were divided into three groups: group I (control group), experimental group II (treated with fresh mecobalamin), experimental group III (treated with expired mecobalamin). ANOVA based significant results were observed for mice body weight, arterial diameter and its wall thickness in renal histological sections of both experimental groups. Similarly, significant changes were observed in arterial diameter, venous wall thickness and lobule diameter in hepatic histological sections. It was concluded that regular long term intake of fresh mecobalamin results in over dosage based side effects whereas exposure to expired mecobalamin causes renal and hepatic tissues necrosis, along with irregular variations of vasodilation and vasoconstriction in blood vessels. Thus, a programmed general public campaign is required to develop awareness that mecobalamin should be used according to prescribed dosage rather than self-medication and before use, the expiry date should be checked and expired drugs should be strictly prohibited to avoid physiological disturbances.