Abstract
The aim of the present paper was to clarify if the prostaglandin F 2α (PGF 2α) production stimulated by mammalian gonadotropin-releasing hormone (mGnRH) comes from arachidonic acid (AA) freed by diacylglyrecol (DAG) and/or membrane phospholipids in the interrenal of Rana esculenta. Interrenals of Rana esculenta were incubated with inhibitors of phospholipase A 1 (PLA 1), phospholipase A 2 (PLA 2), phospholipase C (PLC), protein kinase C (PKC) and diacylglycerol lipase (DAGlipase) in the presence or absence of mGnRH. In parallel, the same experiments were carried out using [ 3H]AA-labelled interrenals. The results of the experiments with non-labelled and [ 3H]AA-labelled interrenals were in agreement. PLA 1, PLA 2, PLC, PKC and DAGlipase inhibitors induced a decrease in PGF 2α production in interrenals without mGnRH, and PLA 2 inhibitor was more effective than other inhibitors. PLC and DAGlipase inhibitors decreased the PGF 2α production by interrenals incubated with mGnRH, and PLC inhibitor was more effective than DAGlipase inhibitor. These findings suggest that the main source of AA used for mGnRH-induced PGF 2α synthesis is DAG; probably this decapeptide increases PGF 2α production enhancing the DAGlipase activity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
