Mammalian Cell Biotechnology in Protein Production

Abstract

Part 1 Genetic manipulation of mammalian cells: Heterologous expression of genes in mammalian cells, Hauser Permanent gene expression in mammalian cells - gene transfer and selection, Schlokar Vector for gene transfer and expression in animal cells, Sandig et al Aspects of gene transfer and gene amplification in recombinant mammalian cells, Wurm Isolation of recombinant cell clones exhibiting high-level expression of the introduced gene, Wirth Genetic engineering of antibodies and derivatives from mammalian cells,Weidle Safety evaluation of products derived from mammalian cell lines, Onions. Part 2 Biological aspects of animal cells: Metabolic control of animal cell culture processes, Wagner Glycosylation - a post-translational modification, Savage. Part 3 Cell cultivation technology: Bioreactors designed for animal cells, Tokashiki, Yokoyama Hydrodinamic properties in bioreactors, Chattopadhyay et al Kinetics and simulation of animal cell processes, Goergen et al On-line and Off-line process analysis, Scheper et al. Part 4 Down-stream processing: Principles of product extraction from cell culture and purification for pharmaceutical proteins, Walter, Allgaier.