Abstract
Cyclodextrin glycosyltransferase catalyses the synthesis of cyclodextrins from starch by intramolecular transglycosylation. However, under certain conditions, the reaction can be directed towards the production of linear dextrins, suppressing the synthesis of cyclodextrins. Thus, the starch acts as donor molecule and low molecular weight oligosaccharides act as acceptors in intermolecular transglycosylation reactions. In this paper the optimal conditions to transform starch into maltooligosaccharides were determined employing glucose and maltose as acceptors and cyclodextrin glycosyltransferase from Bacillus circulans DF 9R as catalyst. Maltooligosaccharides production was optimised in a batch process reaching a yield of 60%. Besides, a continuous process was developed in a membrane reactor, increasing the yield to 72% and multiplying eight times the productivity. In this way, it was possible to obtain a high purity product with higher efficiency, using a cyclodextrin glycosyltransferase. This is even more advantageous when compared with the processes currently used in industry which uses three enzymes.
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