Male and Female Prolactin Receptor mRNA Expression in the Brain of a Biparental and a Uniparental Hamster, Phodopus, Before and After the Birth of a Litter

Abstract

Prolactin receptor (PRL-R) mRNA transcript level was quantified in the choroid plexus (ChP) of a naturally biparental hamster, Phodopus campbelli, and its otherwise similar, yet nonpaternal, sibling species, Phodopus sungorus. Pair-housed males and females on the day before the birth of their first litter (G17), the day after birth (L1), lactation day 5 (L5), and unpaired animals that were sexually naïve, were tested. PRL-R mRNA transcript level relative to total RNA, was evaluated by reverse transcriptase-polymerase chain reaction using primers common to the long- and short-form of the PRL-R in Phodopus. In the ChP, a region implicated in prolactin transport into the central nervous system, females had the expected increase in PRL-R mRNA transcript from dioestrus to L5, consistent with known actions of prolactin. As predicted, males and females of the biparental species were similar, although PRL-R mRNA in naive males was higher than in dioestrus females. Males of the two species also differed as predicted. PRL-R mRNA transcript levels were higher in the biparental males. In addition, P. campbelli males had low PRL-R mRNA at G17 compared to L5. By contrast, non-paternal P. sungorus males had elevated PRL-R mRNA transcript levels on G17 relative to unpaired males. We conclude that PRL-R mRNA in the ChP is differentially regulated before and after birth in a paternal and a nonpaternal male.