Abstract
Mass spectrometry has been gradually adopted by veterinary diagnostic laboratories as the gold standard for microbial species identification. The first part of this chapter aims to review the impact of MALDI-TOF MS in the veterinary microbiology diagnostic laboratory, focusing on the benefits it has brought to reduce the timeline of reporting culture results, the potential for early detection of antimicrobial resistance and the positive impact on biosecurity and infection control in veterinary hospital environments. Second, campylobacteriosis and salmonellosis are the most common foodborne gastrointestinal infections in humans worldwide, and Campylobacter spp. and Salmonella spp. are pathogens that are monitored in surveillance and eradication programmes. Consequently, the possibilities and limitations of identifying these two pathogens using MALDI-TOF MS in the daily routine of veterinary microbiology laboratories are summarized here. Finally, the third part of this chapter focuses on the laboratory diagnosis of animal mycoplasmas, a highly diverse group of unusual bacteria comprising well-recognized pathogens infecting different animal species. Recent studies have shown that MALDI-TOF MS represents a serious alternative to the cumbersome diagnostics of animal mycoplasmas practised so far.
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