Malate-aspartate and alpha-glycerophosphate shuttle enzyme levels in human skeletal muscle: methodological considerations and effect of endurance training.

Abstract

The aim of the present study was to investigate whether the levels of the malate-aspartate and alpha-glycerophosphate shuttle enzymes in human skeletal muscle are affected by endurance training. The approach used was to compare six untrained and six endurance-trained subjects as well as through performing a longitudinal study of endurance training on eight untrained subjects. Biopsy samples were obtained from the lateral part of the quadriceps femoris muscle. The trained muscles were characterized by higher levels of oxidative enzymes (55%) as well as enhanced capillary supply (30%). In both the cross-sectional and longitudinal studies, the malate-aspartate shuttle enzyme levels were about 50% higher in the trained state (cytoplasmic malate dehydrogenase 36%, mitochondrial malate dehydrogenase 46%, cytoplasmic aspartate aminotransferase 52% and mitochondrial aspartate aminotransferase 48%). Contrary to this, the alpha-glycerophosphate shuttle enzyme levels did not differ significantly (cytoplasmic and mitochondrial glycerol-3-phosphate dehydrogenase: 10 and -4%, respectively). The activity ratios of the enzymes involved in respective shuttle did not differ significantly between the untrained and endurance-trained states. It is concluded that endurance training may induce increased levels of malate-aspartate shuttle enzymes in human skeletal muscle while the levels of the alpha-glycerophosphate shuttle enzymes are not affected. The study also includes results from several methodological experiments.