Malate dehydrogenase and lactate dehydrogenase in snail (helix pomatia) foot muscle extract. Comparison of the activity with NADH and deamino-NADH

Abstract

Non specific adenylate deaminase from snail foot muscle deaminated adenylic acid as well as ADP, ATP and NADH at micromolar concentrations to the same extent (1). Malate dehydrogenase activity in 12000xg snail foot muscle extract was 2–6 times higher with deaminoNADH than with NADH. Lactate dehydrogenase activity was 150–250% higher with deaminoNADH than with NADH. The results suggest that nonspecific adenylate deaminase present in snail muscle can cause significant metabolic effects via NADH-dependent dehydrogenases by producing deaminoNADH.