Abstract
Materials and methods During April 2011-November 2012, 1564 patients aged >5 years were included in a larger fever study from seven secondary hospitals in India located in Assam (Tezpur), Bihar (Raxaul), Chhattisgarh (Mungeli), Maharashtra (Ratnagiri), Andhra Pradesh (Anantapur) and Tamil Nadu (Oddanchatram and Ambur). Routine microscopy, genus-specific mitochondrial PCR and the rapid immunochromatographic test (RDT) Parahit TotalTM (Span Diagnostics Ltd, Surat, India) were performed. Species-specific 18S PCR or sequencing was performed on genus PCR positive samples. Samples with discordancy between PCR and RDT were retested by PCR from the extraction step. PCR was considered as gold standard.
Highlights
The World Health Organization reported approximately one million malaria cases and 500 deaths in India in 2012 based on national surveillance data
Materials and methods During April 2011-November 2012, 1564 patients aged >5 years were included in a larger fever study from seven secondary hospitals in India located in Assam (Tezpur), Bihar (Raxaul), Chhattisgarh (Mungeli), Maharashtra (Ratnagiri), Andhra Pradesh (Anantapur) and Tamil Nadu (Oddanchatram and Ambur)
EDTA blood for PCR was available from 1416 patients and 19% were malaria PCR positive
Summary
The aim of this study was to identify the malaria prevalence among patients with acute undifferentiated fever in rural hospitals in India
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