Abstract
In this study, the peroxidase-like activity of macroporous carbon manufactured using a silica template was investigated. The nanozyme activity of macroporous carbon was compared to commercial graphene oxide. The field emission scanning electron microscopy image of carbon revealed macroporous morphology. The nanozyme activity was studied via the catalytic oxidation of chromogenic substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) in the presence of hydrogen peroxide and the oxidized form of ABTS with a green color can be visualized by the eyes. Without functionalization and enzyme utilization, the fabricated macroporous carbon demonstrated green color development, indicating its peroxidase activity probably due to the large surface area and, thus, abundant active sites present on the surface. The oxygen-containing functional groups formed during carbonization act as active sites and can play a pivotal role in the peroxidase-mimicking activity.
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