Making quantal analysis more convenient, fast, and accurate: User-friendly software QUANTAN

Abstract

Quantal analysis of synaptic transmission is an important tool for understanding the mechanisms of synaptic plasticity and synaptic regulation. Although several custom-made and commercial algorithms have been created for the analysis of spontaneous synaptic activity, software for the analysis of action potential evoked release remains very limited. The present paper describes a user-friendly software package QUANTAN which has been created to analyze electrical recordings of postsynaptic responses. The program package is written using Borland C++ under Windows platform. QUANTAN employs and compares several algorithms to extract the average quantal content of synaptic responses, including direct quantal counts, the analysis of synaptic amplitudes, and the analysis of integrated current traces. The integration of several methods in one user-friendly program package makes quantal analysis of action potential evoked release more reliable and accurate. To evaluate the variability in quantal content, QUANTAN performs deconvolution of the distributions of amplitudes or areas of synaptic responses employing a ridge regression method. Other capabilities of QUANTAN include the analysis of the time-course and stationarity of quantal release. In summary, QUANTAN uses digital records of synaptic responses as an input and computes the distribution of quantal content and synaptic parameters. QUANTAN is freely available to other scholars over the internet.