Abstract
Nanoemulsions (NEs) have been made for improving the delivery and disperse of bioactive compounds. In this study, it was found that the best ingredients for the stable Massoia lactone-loaded and food-grade NEs making were 560.0 µL of Tween-80, 240.0 µL of Span-80 and 200.0 µL of Massoia lactone. Then, 9.0 mL of distilled water was titrated into the mixture under continuous magnetic stirring (750 rotations min−1) with about 2 drops per second for 20 min. Finally, the system was treated by ultrasonication using an ultrasonic generator (180 W and 22 KHz) for 5 min. All the prepared particles with a mean droplet diameter of 43 nm were spherical, had uniform size distribution and were equally distributed in the Massoia lactone-loaded NEs. The obtained Massoia lactone-loaded nanoemulsions (NEs) were very stable without changes of the mean droplet diameter and polydispersity indexes (PDI) for over two months under different conditions. As with free Massoia lactone, Massoia lactone loaded in the NEs had high anti-fungal activity against Metschnikowia bicuspidate LIAO, a pathogenic yeast causing milky disease in the Chinese mitten crab by damaging its cell membrane and causing cellular necrosis. Massoia lactone loaded in the NEs also had the DPPH radical scavenging activity and the hydroxyl radical scavenging activity.
Highlights
IntroductionMassoia lactone used in this study is an α, β-unsaturated δ-lactone moiety substituted at the C6 position by an alkyl chain of 5 carbons
It has been found that a large amount of Massoia lactone can be obtained by hydrolysis of liamocins which are synthesized by different strains of marine-derived Aureobasidium spp. and are composed of a single mannitol or arabitol or xylitol headgroup linked to either three or four or even six
It has been reported that nonionic surfactants such as Tween and Span with a hydrophile lipophilic balance (HLB) value greater than 8 could be successfully used to make the oil in water (O/W)
Summary
Massoia lactone used in this study is an α, β-unsaturated δ-lactone moiety substituted at the C6 position by an alkyl chain of 5 carbons. It can be released from liamocins synthesized and secreted by the marine-derived Aureobasidium spp. and it has been clear how to biosynthesize liamocins at the molecular level [1]. Because such Massoia lactone has many bioactivities and can be used as a flavoring and fragrant agent, it can be widely applied to food and flavor industries [1], indicating that it is safe to humans. It has been found that a large amount of Massoia lactone can be obtained by hydrolysis of liamocins which are synthesized by different strains of marine-derived Aureobasidium spp. and are composed of a single mannitol or arabitol or xylitol headgroup linked to either three or four or even six
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
