Major metabolite of F2-isoprostane in urine may be a more sensitive biomarker of oxidative stress than isoprostane itself

Abstract

There is limited literature on the contributors to isoprostane metabolite 2,3-dinor-5,6-dihydro-15-F(2t)-isoprostane (15-F(2t)-IsoP-M) compared with F(2)-isoprostanes (F(2)-IsoPs) as an oxidative stress biomarker. The objective of this study was to investigate whether plasma concentrations of antioxidants, urinary excretion rates of polyphenols, and antioxidants in food and dietary supplements are attributable to both urinary F(2)-IsoP and 15-F(2t)-IsoP-M concentrations. Dietary intake information and blood and urine samples were obtained from 845 healthy middle-aged and elderly female participants of the Shanghai Women's Health Study. Urinary isoprostanes (F(2)-IsoPs and 15-F(2t)-IsoP-M) were measured and adjusted for creatinine concentrations. Urinary 15-F(2t)-IsoP-M and F(2)-IsoP concentrations were lower in subjects who used a multivitamin. Lower F(2)-IsoP concentrations were observed in ginseng users, whereas lower concentrations of 15-F(2t)-IsoP-M were shown in subjects who used a vitamin E supplement. Plasma concentrations of several antioxidants (ie, β-carotenes, both trans and cis β-carotenes, lycopene other than trans, 5-cis and 7-cis isomers, cis anhydrolutein, and cis β-cryptoxanthin) were inversely associated with 15-F(2t)-IsoP-M but not with F(2)-IsoPs, whereas β-, γ-, and δ-tocopherols were positively associated with 15-F(2t)-IsoP-M but not with F(2)-IsoPs. Urinary polyphenol quercetin was positively associated with both F(2)-IsoPs and 15-F(2t)-IsoP-M. The results suggest that the F(2)-IsoP major metabolite 15-F(2t)-IsoP-M may be a more sensitive marker of endogenous oxidative stress status than are F(2)-IsoPs in the assessment of effects of antioxidants on age-related diseases.