Abstract
Genetic transformation of maize via Agrobacterium tumefaciens is still more art than science, with different researchers achieving substantially different transformation results. This article describes our advanced Agrobacterium-mediated transformation system in Hi-II maize. The system utilizes simple binary vectors and immature embryos for the transformation, employing the bar gene as a plant selectable marker in combination with bialaphos for subsequent culture selection. The transformation process is efficient and highly reproducible. Certain inbred maize lines can also be transformed with some modification of the system. © 2016 by John Wiley & Sons, Inc.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
