Abstract

Maize kernel traits such as kernel length, kernel width, and kernel number determine the total kernel weight and, consequently, maize yield. Therefore, the measurement of kernel traits is important for maize breeding and the evaluation of maize yield. There are a few methods that allow the extraction of ear and kernel features through image processing. We evaluated the potential of deep convolutional neural networks and binary machine learning (ML) algorithms (logistic regression (LR), support vector machine (SVM), AdaBoost (ADB), Classification tree (CART), and the K-Neighbor (kNN)) for accurate maize kernel abortion detection and classification. The algorithms were trained using 75% of 66 total images, and the remaining 25% was used for testing their performance. Confusion matrix, classification accuracy, and precision were the major metrics in evaluating the performance of the algorithms. The SVM and LR algorithms were highly accurate and precise (100%) under all the abortion statuses, while the remaining algorithms had a performance greater than 95%. Deep convolutional neural networks were further evaluated using different activation and optimization techniques. The best performance (100% accuracy) was reached using the rectifier linear unit (ReLu) activation procedure and the Adam optimization technique. Maize ear with abortion were accurately detected by all tested algorithms with minimum training and testing time compared to ear without abortion. The findings suggest that deep convolutional neural networks can be used to detect the maize ear abortion status supplemented with the binary machine learning algorithms in maize breading programs. By using a convolution neural network (CNN) method, more data (big data) can be collected and processed for hundreds of maize ears, accelerating the phenotyping process.

Highlights

  • Maize (Zea mays L.) productivity is strongly related to the number and mass of harvested kernels which are the key grain yield determinants

  • There are few methods that allow the extraction of ear and kernel attributes through image processing

  • Kernel abortion represents a major challenge to the extraction of maize ear and kernel features through image processing

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Summary

Introduction

Maize (Zea mays L.) productivity is strongly related to the number and mass of harvested kernels which are the key grain yield determinants. One was patented by Pioneer [10] It enables to extract kernel count, kernel size distribution, proportion of aborted kernels, and other information using image processing algorithms that include, without limitation, filtering, water shedding, thresholding, edge finding, edge enhancement, color selection, and spectral filtering. Kernel abortion represents a major challenge to the extraction of maize ear and kernel features through image processing Traditional approaches such as bio-physical modeling struggle to attain high precision and accuracy in modeling and processing images with and without abortion [12]. In addition to all the above advantages, machine learning has limitations mostly related to the sets to train on,for asmassive, well as time and resources needed to achieve reasonable relevancy requirements inclusive/unbiased, and good quality data sets to accuracy train on, and as well as time and error-susceptibility.

Methods
Image Embedding
RGB layersasmeans
Convolutional Neural Networks
Binary Classification Machine Learning Algorithms
General
ROC Analysis
Calibration Plot
Binary Classification Algorithms
Calibration
Deep Convolutional Neural Network
Conclusions
Full Text
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