Abstract
The primary symbiote of the pea aphid was maintained in liquid culture media for 12 hr for the purpose of optimizing certain culture parameters. The optimum temperature for protein and nucleic acid synthesis was found to be 28–30°C. There was no dependence of this microorganism on fetal calf serum added to the growth medium. Grace's insect tissue culture medium and Grace's insect tissue culture medium (modified by Tokumitsu and Maramorosch) were found to be best suited for the maintenance of this organism. The suitability of the growth medium for the microorganism was directly related to the age of the culture medium.
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