Abstract
1. 1. Various systems for the maintenance of rat adipose tissue in tissue culture have been compared. 2. 2. Optimal conditions for tissue culture were medium 199 supplemented with insulin (10 μg/ml). streptomycin (10μ/ml). penicillin (6 μg/ml) and buffered with 25 mM Hepes. pH 7.3. with a gas phase of air and a temperature of 33 C; adipose tissue was either placed on grids at the air-liquid interface or was allowed to float on the liquid, depending on the age of the rat from which it came. 3. 3. The rates of glucose metabolism to CO 2, fatty acids and glyceride glycerol. and also palmitate esterification in adipose tissue slices changed (but not in synchrony) over 3 days in tissue culture. 4. 4. It is concluded that the system should be of value for studying effects of hormones and other substances on adipose tissue metabolism over a period of several days.
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