Abstract
1. 1. Adult Litomosoides carinii have been maintained in vitro for 23 days and the females have produced microfilariae for 18 days. The cultures were kept at 37 °C in a medium containing fresh rat serum and Parker 199. The average numbers of microfilariae produced by female L. carinii, per day, in vitro, ranged from 1,000 to 8,600, in different experiments. 2. 2. Adult Dirofilaria immitis have been maintained in vitro for 8 days but the females continued the production of microfilariae for only the first two days. 3. 3. Microfilaria immitis, Mf. loa and Mf. bancrofti have survived in culture for from 10 to 14 days; the G cells 1 and 2 of Mf. loa divided under these conditions. 4. 4. Infective larvae of D. immitis remained alive and healthy for 9 days in vitro. They moulted during this time but did not increase in size.
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