Magnoflorine from Tinospora crispa upregulates innate and adaptive immune responses in Balb/c mice

Abstract

Magnoflorine shows a diverse range of pharmacological actions, including immunomodulatory, antioxidant and neuropharmacological activities. However, its effects on the immune responses in animal studies have not been reported. In this study, magnoflorine isolated from Tinospora crispa, at doses of 25, 50 and 100 mg/kg was administered to male Balb/c mice daily for 14 days to evaluate its effect on innate immune responses, while for evaluation of adaptive immune responses, on day 0 the mice were injected intraperitoneally with sheep red blood cells (sRBC) and treated orally with the various doses of magnoflorine for the same duration. The effects of magnoflorine on phagocytosis, myeloperoxidase (MPO) activity, lysozyme serum level, nitric oxide (NO) production, CD4+ and CD8+ cells population, T and B lymphocytes proliferation, activated T cells cytokines production, antibodies levels and delayed type hypersensitivity (DTH) were determined. Magnoflorine dose-dependently stimulated NO production, E. coli engulfment by neutrophils and peritoneal macrophages, MPO activity and lysozyme serum level in treated mice. Magnoflorine at 100 mg/kg exhibited comparable stimulation of B cell production compared to levamisole at 2.5 mg/kg. It also significantly increased CD4+ and CD8+ cells population, upregulated the Th1 (IFN-γ, IL-2 and TNF-α) and Th2 (IL-4 and IL-6) cytokines in a dose-dependent manner. At similar concentrations, magnoflorine also exhibited a strong dose-dependent stimulation on DTH reaction and upregulation of immunoglobulins (IgG and IgM) production in mice immunized with sRBC. The strong upregulation of innate and adaptive immune responses indicates that magnoflorine has potential to be developed into an effective immunostimulant.