Abstract
In this paper, we present a new magnetophoretic cell separation method based on the magnetic susceptibility differences between cells and medium. Since the dimension of cells is only a few dozen micrometers, it is difficult to discriminate cells only by low magnetic force acting on biological cells. To improve the efficiency of this label-free magnetophoretic separation, the magnetic susceptibility of surrounding solution is adjusted by dissolving paramagnetic salt such as gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA), in which cells experiences an enhanced magnetic repulsive force. As the concentration of Gd-DTPA increases, the difference in the magnetic force acting on both polystyrene beads and cells increases, resulting in more efficient separation. Based on these results, we have successfully achieved label-free separation of U937 cells from red blood cells with >; 90% purity and 1 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sup> cells/h throughput.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
