Magnetite modified zeolite as an alternative additive to promote methane production from anaerobic digestion of waste activated sludge

Abstract

Methane recovery from bio-wastes is one promising renewable energy to relieve the energy crisis faced around the world. Zeolite is one of attractive exogenous additives for promoting methane production from anaerobic digestion due to its potentials in regulating microorganism enrichment and anaerobic environment. However, the poor electron mediated ability limited its positive effects. Hence, this study hypothesized that the electron mediated ability of zeolite would be improved by loading magnetite, thereby generating more positive impacts on the performance of sludge anaerobic digestion. Compared to control group, magnetite modified zeolite (MZ) effectively improved methane yield by 37.4%. The reason was that the electron mediated ability and adsorption capacity of zeolite were improved by loading magnetite, facilitating the degradation of refractory organics and the conversion of volatile fatty acids. Moreover, the activities of cytochrome c and F420 increased by 33.3% and 29.8% with MZ additive, contributing to stimulating efficient electron transfer process. In addition, the electro-active genera were enriched, such as Bacteroidetes_vadinHA17, Methanobacterium, and Methanosaeta, and the direct interspecies electron transfer was possibly established for methanogenesis with the help of MZ. This study will provide valuable references for the application of zeolite in anaerobic digestion.