Magnetite Beads Binding to Human Serum Protein

Abstract

Serum biomarker analysis of blood protein is often interfered with by the presence of dominant protein fractions such as albumin and globulin when analytical separation is performed. Magnetite solid phase extraction (MSPE) has been shown to be fast, effective and efficient to enable cleanup of proteins. Some studies have reported that magnetite adsorption to albumin is about 40-90% in a pure solution after several hours mixing and homogenizing. Few studies have examined the binding of magnetite to total protein in a human blood matrix. The magnetite coprecipitation method was performed in this study. Coprecipitation of magnetite was carried out by mixing 2:1 FeCl (III):(II) 0.01 M in an aqueous medium for 40 minutes at 90