Magnetic solid phase extraction followed with LC-MS/MS for determination of glimepiride in beagle dog plasma and its application to bioequivalence study

Abstract

In this study, the core-shell polydopamine-coated magnetic nanomaterials were synthesized with one-step approach and applied as magnetic solid phase extraction adsorbents combined with LCMS/MS for quantification of the diabetes drug glimepiride in beagle dog plasma. The Fe3O4@PDA nanomaterials have strong magnetic response, good dispersibility in aqueous solution, and numerous binding sites for π–π interaction and hydrogen bonding with glimepiride. Based on these merits, glimepiride could be quickly extracted and separated from plasma within 10min. The established method has good linearity (1-2000ng•mL−1), low quantification limit (1ng•mL−1), good precision (RSDs≤12 %), and satisfactory extraction recovery (71.20–85.70 %). Moreover, we successfully applied this method to the bioequivalence study of generic and innovator products of glimepiride in beagle dog plasma.