Abstract
MRI for in vivo stem cell tracking remains controversial. Here we tested the hypothesis that MRI can track the long-term fate of the superparamagnetic iron oxide (SPIO) nanoparticles labelled mesenchymal stem cells (MSCs) following intramyocardially injection in AMI rats. MSCs (1 × 106) from male rats doubly labeled with SPIO and DAPI were injected 2 weeks after myocardial infarction. The control group received cell-free media injection. In vivo serial MRI was performed at 24 hours before cell delivery (baseline), 3 days, 1, 2, and 4 weeks after cell delivery, respectively. Serial follow-up MRI demonstrated large persistent intramyocardial signal-voids representing SPIO during the follow-up of 4 weeks, and MSCs did not moderate the left ventricular dysfunction. The TUNEL analysis confirmed that MSCs engrafted underwent apoptosis. The histopathological studies revealed that the site of cell injection was infiltrated by inflammatory cells progressively and the iron-positive cells were macrophages identified by CD68 staining, but very few or no DAPI-positive stem cells at 4 weeks after cells transplantation. The presence of engrafted cells was confirmed by real-time PCR, which showed that the amount of Y-chromosome-specific SRY gene was consistent with the results. MRI may not reliably track the long-term fate of SPIO-labeled MSCs engraftment in heart.
Highlights
{ Current address: Arrhythmia Diagnosis and Treatment Center, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College
Studies have reported that MRI can track the engrafted mesenchymal stem cells (MSCs)’ fate in vivo after these cells were labeled with superparamagnetic iron oxide (SPIO), and SPIO do not affect cell viability, proliferation, differentiation or migration[10,11]
MSCs were grown in complete culture medium containing 25 mg/mL SPIO (Feridex IV, Advanced Magnetics) and 0.375/ mL poly-L-lysine (Sigma) in a humidified 5% CO2 incubator at 37uC for 48 h and followed by washing three times in phosphate-buffered saline (PBS) to rinse off SPIO particles on the surface of cells
Summary
{ Current address: Arrhythmia Diagnosis and Treatment Center, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College. MRI for in vivo stem cell tracking remains controversial. We tested the hypothesis that MRI can track the long-term fate of the superparamagnetic iron oxide (SPIO) nanoparticles labelled mesenchymal stem cells (MSCs) following intramyocardially injection in AMI rats. MRI may not reliably track the long-term fate of SPIO-labeled MSCs engraftment in heart. Studies have reported that MRI can track the engrafted MSCs’ fate in vivo after these cells were labeled with SPIO, and SPIO do not affect cell viability, proliferation, differentiation or migration[10,11]. The aim of this study was to determine whether MRI can track the long-term fate of the SPIO nanoparticles labeled adult rat MSCs including survival and migration in rat models of myocardial infarction following intramyocardially injection
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