Magnetic relaxation switch sensor based on aptamer-modified poly-L-lysine-ferroferric oxide magnetic nanoparticles and graphene oxide for the determination of insecticides in vegetables.

Abstract

A simple and effective graphene oxide-magnetic relaxation switch (GO-MRS) sensor that combines graphene oxide (GO) and aptamer-modified poly-L-lysine(PLL)-Fe3O4 nanoparticles (Fe3O4@PLL-Apt NPs) was designed for the detection of acetamiprid (ACE). In this sensor, Fe3O4@PLL-Apt NPs acted as a relaxation signal probe and GO facilitated the generation of relaxation signal changes (dispersion/aggregation shift), while the aptamer is a molecular component that recognizes ACE. This GO-assisted magnetic signal probe improves the stability of magnetic nanoparticles in solution and enhances their sensitivity to small molecules while avoiding cross-reactions. Under optimal conditions, the sensor exhibits a wide working range (10-80nM) and low detection limit (8.43nM). The spiked recoveries ranged from 96.54 to 103.17%, with a relative standard deviation (RSD) of less than 2.3%. In addition, the performance of the GO-MRS sensor matched that of the standard method (liquid chromatography-mass spectrometry (LC-MS)), indicating that the GO-MRS sensor is suitable for the detection of ACE in vegetables.