Magnetic Properties of Iron Oxide Nanoparticles Do Not Essentially Contribute to Ferrogel Biocompatibility.

Felix A Blyakhman,Pavel A Shabadrov,Fedor A Fadeyev,Tatyana F Shklyar,Galina V Kurlyandskaya,Sergio Fernandez Armas,Emilia B Makarova,Alexander P Safronov

doi:10.3390/nano11041041

Felix A Blyakhman, Pavel A Shabadrov + Show 6 more

Open Access

https://doi.org/10.3390/nano11041041

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Abstract

Two series of composite polyacrylamide (PAAm) gels with embedded superparamagnetic Fe2O3 or diamagnetic Al2O3 nanoparticles were synthesized, aiming to study the direct contribution of the magnetic interactions to the ferrogel biocompatibility. The proliferative activity was estimated for the case of human dermal fibroblast culture grown onto the surfaces of these types of substrates. Spherical non-agglomerated nanoparticles (NPs) of 20–40 nm in diameter were prepared by laser target evaporation (LTE) electrophysical technique. The concentration of the NPs in gel was fixed at 0.0, 0.3, 0.6, or 1.2 wt.%. Mechanical, electrical, and magnetic properties of composite gels were characterized by the dependence of Young’s modulus, electrical potential, magnetization measurements on the content of embedded NPs. The fibroblast monolayer density grown onto the surface of composite substrates was considered as an indicator of the material biocompatibility after 96 h of incubation. Regardless of the superparamagnetic or diamagnetic nature of nanoparticles, the increase in their concentration in the PAAm composite provided a parallel increase in the cell culture proliferation when grown onto the surface of composite substrates. The effects of cell interaction with the nanostructured surface of composites are discussed in order to explain the results.

Highlights

In the past several decades, a number of biologically inert materials were introduced as useful composites for cell culturing for the needs of tissue engineering [1,2]
We tested the biocompatibility of ferrogel based on the polyacrylamide (PAAm) polymer network with embedded maghemite (γ-Fe2 O3 ) magnetic nanoparticles (MNPs) fabricated by the electrophysical technique of the laser target evaporation (LTE) [11]
It became clear that the addition of magnetic nanoparticles to the PAAm gel network always resulted in an increase in cell adhesion and proliferation when grown onto the surface of the gel-based composites [12,13]

Summary

Introduction

In the past several decades, a number of biologically inert materials were introduced as useful composites for cell culturing for the needs of tissue engineering [1,2]. We tested the biocompatibility of ferrogel based on the polyacrylamide (PAAm) polymer network with embedded maghemite (γ-Fe2 O3 ) magnetic nanoparticles (MNPs) fabricated by the electrophysical technique of the laser target evaporation (LTE) [11]. Such parameters as adhesion and proliferation of human peripheral. It became clear that the addition of magnetic nanoparticles to the PAAm gel network always resulted in an increase in cell adhesion and proliferation when grown onto the surface of the gel-based composites [12,13]. The gradual increase in magnetic nanoparticle concentration in PAAm gel from 0 to 2 wt.% was accompanied by the increase in cell monolayer density by a factor of five for the culture grown onto the FG surface [14]

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