Abstract
Magnetic porous molecularly imprinted polymers (MPMIPs) for rapid and efficient selective recognition of chlorogenic acid (CGA) were effectively prepared based on surface precipitation polymerization using CGA as template, 4-vinylpyridine (4-VP) as functional monomer, and mesoporous SiO2 (mSiO2) layer as sacrificial support. A computational simulation by evaluation of electronic binding energy is used to optimize the stoichiometric ratio between CGA and 4-VP (1:5), which reduced the duration of laboratory trials. The porous MIP shell and the rid of solid MIPs by magnet gave MPMIPs high binding capacity (42.22 mg/g) and fast kinetic binding (35 min). Adsorption behavior between CGA and MPMIPs followed Langmuir equation and pseudo-first-order reaction kinetics. Furthermore, the obtained MPMIPs as solid phase adsorbents coupled with high performance liquid chromatography (HPLC) were employed for selective extraction and determination of CGA (2.93 ± 0.11 mg/g) in Duzhong brick tea. The recoveries from 91.8% to 104.2%, and the limit of detection (LOD) at 0.8 μg/mL were obtained. The linear range (2.0–150.0 μg/mL) was wide with R2 > 0.999. Overall, this study provided an efficient approach for fabrication of well-constructed MPMIPs for fast and selective recognition and determination of CGA from complex samples.
Highlights
Quantification of trace compounds in complex matrices is always a big analytical challenge.In order to extract target compounds and get rid of interferences for accurate and sensitive analysis, extraction methods have been emerged [1,2,3]
Magnetic porous molecularly imprinted polymers (MPMIPs)-based solid phase extraction coupled with high performance liquid chromatography (HPLC) were developed and validated for selective determination of chlorogenic acid (CGA) in Duzhong brick tea
The results indicated the successful preparation of MPMIPs
Summary
Quantification of trace compounds in complex matrices is always a big analytical challenge. Different types of sorbents have been developed for solid-phase extraction, such as C8 /C18 -bonded silica [4], carbon materials [5], polymeric ionic liquids [6], metal–organic frameworks [7], mesoporous or nanoporous silica [8], molecularly imprinted polymers (MIPs) [9,10,11,12], etc. To our best knowledge, no paper has reported the preparation of MPMIPs for effective and selective extraction of CGA from complex matrices. In the present study, with the theoretical calculation results, MPMIPs, using Fe3 O4 as magnetic core, and porous CGA imprinted MIPs as shell, were prepared for the first time. MPMIP-based solid phase extraction coupled with high performance liquid chromatography (HPLC) were developed and validated for selective determination of CGA in Duzhong brick tea
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