Abstract
Nanotechnologies involving the use of magnetic nanoparticles made of nickel, iron or cobalt are nowadays developing in anti-tumor therapy and diagnostic. The most important potential of MNPs is for drug delivery, meaning they are functionalized by binding to chemotherapeutic agents, nucleic acids, antibodies, etc. The present study is questioning the possibility of oleic acid-coated iron MNPs to induce cell death per se, selectively in tumor cells (breast cancer adenocarcinoma SK-BR3 cell line) without previous coupling with effector substances.
Highlights
Nanotechnologies involving the use of magnetic nanoparticles made of nickel, iron or cobalt are nowadays developing in anti-tumor therapy and diagnostic
The SK-BR3 cells treated with 10 mM magnetite nanoparticles (MNPs) lost their adherence ability after 24h in contact with MNPs, and we were able to visualize the nuclei pushed to a peripheral part of the cell, close to cell membrane, while the cell had a round morphology
MNPs, on average 90% of SK-BR3 cells exhibited the loss of nucleus
Summary
American Type Culture Collection (ATCC, Manassas, VA, USA) and the cells were expanded in McCoy’s 5A medium (Gibco BRL, Invitrogen, Carlsbad, CA, USA) enriched with. Streptomycin mixture (Pen/Strep, 10000 IU/mL; PromoCell, Heidelberg, Germany). Cellular maintenance was in standard incubator conditions atmosphere. 5a0t0307°cCe,ll5s/%cmCO2 w2,earnedsheuemdeidd in 6-well plates for microscopic investigations, while for viability testing, the cells were plated in specific 16-well E-. Prior to MNPs addition, the cells were left overnight (24h) to attach. MNPs synthesis The magnetic nanoparticles (MNPs) were synthetized bwyhiccohmwbeursetifounrthmeer tdhooudb,lege-lnayeerartcinogatFeed3Ow4ithnaonleoipcoawcidde, rass, described by Ianos R et al and dispersed in Phosphate Buffer Saline (PBS, St. Louis, Missouri, USA) [7]
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