Abstract
A novel membrane-based lateral-flow immunodipstick assay was developed for the fast screening of aflatoxin B2 (AFB2) as a model compound in food samples. The detector reagent consisted of magnetic nanogold microspheres (MnGMs) with nano-Fe2O3 particles as core and gold nanoparticles as shell, and bio-functionalized with monoclonal anti-AFB2 antibodies. Manually spotted AFB2–bovine serum albumin conjugates (AFB2–BSA) and goat anti-mouse IgG on nitrocellulose membrane were used as test and control lines, respectively. As the major advantage, experimental results indicated that the visual detection limit (cutoff value) of the MnGM-based dipstick immunoassay with 0.9ng/ml AFB2 was about threefold lower compared to a conventional immunodipstick test using gold nanoparticles as detection reagent. Qualitative results (yes/no) could be obtained within 15min without expensive equipment. The assay was evaluated with AFB2 spiked or naturally contaminated samples (n=8), including peanuts, hazelnuts, pistacia and almonds, receiving excellent correspondance with results from high performance liquid chromatography (HPLC). Most importantly, the assay gave no false negative results. By controlling the target antibody this assay can be easily extended for use with other food relevant toxins and thus represents a versatile detection method.
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