Magnetic nanofluid based on green deep eutectic solvent for enrichment and determination of chloramphenicol in milk and chicken samples by high-performance liquid chromatography-ultraviolet: Optimization of microextraction

Abstract

In this work, magnetic nanofluid based on a deep eutectic solvent that constricts through a simple and easy route, and subsequently applied for the preconcentration and microextraction of chloramphenicol (CAP) as a hazardous drug from milk and chicken samples via syringe-to-syringe microextraction prior to its determination by high-performance liquid chromatography–ultraviolet (HPLC-UV). In addition, the optimum conditions of effective factors were searched by the central composite design (CCD), and subsequently, at their optimum value, the figures of merit were evaluated. Also, the suggested method illustrated a low limit of detection (0.2 ng mL−1), a low limit of quantitation (0.67 ng mL−1), and a good linear range with an R2 of 0.996. The CAP relative recoveries in milk and chicken samples were 90.3%–95.1%, with relative standard deviations lower than 4.2%. The current enhancement technique is simple, easy, and rapid, which makes it suitable for quantification of CAP by HPLC-UV at trace levels in complicated materials with reliable and reproducible results.