Abstract
目的 应用菲立磁-多聚左旋赖氨酸复合物标记大鼠骨髓间充质干细胞,探讨MR成像显示磁标记干细胞的可行性.方法制备菲立磁-多聚左旋赖氨酸复合物.分离培养Wistar大鼠骨髓间充质干细胞,以菲立磁-多聚左旋赖氨酸复合物标记干细胞.分别于标记后24 h及1、2、3周行普鲁士蓝染色观察细胞内铁,台盼蓝排除试验检测细胞活力.应用1.5 T MR仪,以SE序列T1WI、T2WI和梯度回波(GRE)序列T2*WI行磁标记干细胞成像.结果普鲁士蓝染色显示细胞质内大量铁颗粒存在,标记率100%;随细胞分裂增殖,细胞内铁颗粒逐渐减少.干细胞磁标记后24 h及1、2、3周的台盼蓝拒染率分别为91.00%、93.00%、91.75%和92.50%,与未标记细胞相比较差异无统计学意义(P>0.05). 103、104、105个磁标记干细胞T2WI 信号降低分别为63.75%、82.31%、91.92%, T2*WI信号降低分别为68.24%、83.01%、93.94%.105个干细胞磁标记后24 h及1、2、3周T2*WI信号降低分别为93.75%、75.92%、41.75%、8.83%.结论应用菲立磁-多聚左旋赖氨酸复合物标记大鼠间充质干细胞安全、有效;T2*WI对磁标记干细胞的显示最敏感;MR信号改变与干细胞数目及分裂增殖状态相关。
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