Abstract
One of the main interests in phase plate imaging is motivated by a decrease in irradiation dose needed to obtain desired signal to noise ratio, a result of improved contrast transfer [1]. The decrease in irradiation improves the imaging of biological materials [2]. Here we demonstrate that phase plate imaging of magnetic samples (phase objects), using a hole-free phase plate (HFPP) [3], is superior to conventional Fresnel imaging with significantly improved signal to background ratio under in-focus or near-in-focus conditions.
Highlights
In principle, phase plate imaging should make it possible to image most phase objects, including magnetic and electrostatic fields in vacuum
The requirement for phase plate imaging, including that by hole-free phase plate (HFPP), is that the object spectrum in the back focal plane of the objective lens must not be broadened via the effect of chromatic aberration
The data shown here were obtained on a JEOL 2100 FM-LM microscope, equipped with low-field objective lens that is dedicated for magnetic imaging without affecting a sample's magnetic state
Summary
Phase plate imaging should make it possible to image most phase objects, including magnetic and electrostatic fields in vacuum. The requirement for phase plate imaging, including that by HFPP, is that the object spectrum in the back focal plane of the objective lens must not be broadened via the effect of chromatic aberration. The imaging of magnetic samples, including magnetic field in vacuum, proved possible using a HFPP [4].
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